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KMID : 0358420080510020199
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Korean Journal of Obstetrics and Gynecology
2008 Volume.51 No. 2 p.199 ~ p.211
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The effect of GM-CSF on the expression of implantation-related genes in mouse embryo
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Kim Hyo-Jung
Kim Hye-Min
Kim Jae-Lyung
Shin Jung-Hwan
Hong Seo-Yu
Park Eun-Joo
Park Won-Il
Lee Jin-Yong
Cha Byung-Hun
Moon Hye-Sung
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Abstract
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Objective : GM-CSF is produced in female reproductive tract and may play an important role in the process of implantation. Body of evidence suggests that GM-CSF could improve pregnancy rate in many species of mammals when it was added in culture media. The aim of this study is to assess how GM-CSF affects the expression of implantation-related genes in mouse embryo.
Methods : Two hundred mouse embryos were divided into control and GM-CSF treated groups. The embryos were treated with or without 10 ng/ml of GM-CSF for 72 hours. Total RNA was isolated and compared with oligo microarray. The implantation-related genes influenced by GM-CSF were repeatedly analyzed by real-time PCR.
Results : After oligo microarray, 64 genes were increased and 35 genes were decreased by GM-CSF. Among those genes, MMP2, FABP3, Dppa5 and TAS1 were selected for real-time PCR analysis. Four integrins and FAK were also selected. We confirmed the increase of MMP2 and FABP3 by GM-CSF with real-time RT-PCR (1.687 and 1.580 fold, respectively). Other genes were found to be minimally increased.
Conclusion : GM-CSF induces the increased expression of MMP2 mRNA in mouse embryo, and then increases the invasiveness of the trophoblast. The role of FABP3 in the process of implantation remained to be elucidated.
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KEYWORD
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Embryo, Implantation, GM-CSF, MMP2, FABP3
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